dc.contributor
Institut Català de la Salut
dc.contributor
[Martínez-Sabadell A] Preclinical Research Program, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Cancer Research Program, Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain. Centro de Investigación Biomédica en Red de Cáncer, Madrid, Spain. Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Ovejero Romero P] Preclinical Research Program, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. [Arribas J] Preclinical Research Program, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Cancer Research Program, Institut Hospital del Mar d’Investigacions Mèdiques (IMIM), Barcelona, Spain. Centro de Investigación Biomédica en Red de Cáncer, Madrid, Spain. Department of Medicine and Life Sciences, Universitat Pompeu Fabra, Barcelona, Spain. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. [Arenas EJ] Preclinical Research Program, Vall d’Hebron Institute of Oncology (VHIO), Barcelona, Spain. Centro de Investigación Biomédica en Red de Cáncer, Madrid, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
Ovejero Romero, Pablo
dc.contributor.author
Arribas López, Joaquin Vicente
dc.contributor.author
Arenas Lahuerta, Enrique Javier
dc.contributor.author
Martínez-Sabadell, Alex
dc.date.accessioned
2025-10-24T09:34:24Z
dc.date.available
2025-10-24T09:34:24Z
dc.date.issued
2022-11-28T11:49:01Z
dc.date.issued
2022-11-28T11:49:01Z
dc.date.issued
2022-12-16
dc.identifier
Martínez-Sabadell A, Ovejero Romero P, Arribas J, Arenas EJ. Protocol to generate a patient derived xenograft model of acquired resistance to immunotherapy in humanized mice. STAR Protoc. 2022 Dec 16;3(4):101712.
dc.identifier
https://hdl.handle.net/11351/8546
dc.identifier
10.1016/j.xpro.2022.101712
dc.identifier.uri
https://hdl.handle.net/11351/8546
dc.description.abstract
Cancer; Cell isolation; Stem cells
dc.description.abstract
Càncer; Aïllament cel·lular; Cèl·lules mare
dc.description.abstract
Cáncer; Aislamiento celular; Células madre
dc.description.abstract
Immunotherapy has revolutionized cancer treatment, but preclinical models are required to understand immunotherapy resistance mechanisms underlying patient relapse. This protocol describes how to generate an acquired resistance humanized in vivo model to immunotherapies in patient-derived xenografts (PDX). We detail steps to inject human CD34+ cells into NSG mice, followed by generation of immunoresistant PDX in humanized mice. This approach recapitulates the human immune system, allowing investigators to generate preclinical resistance models to different immunotherapies for identifying the resistant phenotype. For complete details on the use and execution of this protocol, please refer to Martínez-Sabadell et al., 2022 and Arenas et al. (2021).
dc.description.abstract
This work was supported by Asociación Española Contra el Cancer (GCAEC19017ARRI), Breast Cancer Research Foundation (BCRF-21-008), and Instituto de Salud Carlos III (PI19/01181). A.M.S. was funded by the Spanish Government (PFIS FI20/00188). P.O.R. was funded by the BBVA. E.J.A. was funded by the AECC (POSTD211413AREN). VHIO would like to acknowledge the Cellex Foundation for providing research facilities and equipment and the Centro de Investigación Biomédica en Red de Cáncer (CIBERONC) from the Institute of Health Carlos III (ISCIII) for their support on this research. Authors acknowledge financial support for the Cancer Immunology and Immunotherapy (CAIMI-2) program funded by BBVA Foundation. The Graphical abstract was created with BioRender.com.
dc.format
application/pdf
dc.relation
STAR Protocols;3(4)
dc.relation
https://doi.org/10.1016/j.xpro.2022.101712
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Càncer - Immunoteràpia
dc.subject
Trasplantació d'òrgans, teixits, etc.
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Investigative Techniques::Neoplasm Transplantation::Xenograft Model Antitumor Assays
dc.subject
ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Therapeutics::Biological Therapy::Immunomodulation::Immunotherapy
dc.subject
ORGANISMS::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Eutheria::Rodentia::Muridae::Murinae::Mice
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas de investigación::trasplante de neoplasias::ensayos antitumorales por modelo de xenoinjerto
dc.subject
TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::terapéutica::terapia biológica::inmunomodulación::inmunoterapia
dc.subject
ORGANISMOS::Eukaryota::animales::Chordata::vertebrados::mamíferos::Eutheria::Rodentia::Muridae::Murinae::ratones
dc.title
Protocol to generate a patient derived xenograft model of acquired resistance to immunotherapy in humanized mice
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
