Dental pulp of the third molar: a new source of pluripotent-like stem cells

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RECERCAT Home > Universitat Internacional de Catalunya > Recerca > Articles > Ciències de la Salut > View document

To access the full text documents, please follow this link: http://hdl.handle.net/20.500.12328/1234

dc.contributor.author	Atari Abouassi, Maher
dc.contributor.author	Gil Recio, Carlos
dc.contributor.author	Fabregat, Marc
dc.contributor.author	García Fernández, Daniel
dc.contributor.author	Barajas Vélez, Miguel
dc.contributor.author	Carrasco García, Miguel Ángel
dc.contributor.author	Jung, Han-Sung
dc.contributor.author	Hernández Alfaro, Federico
dc.contributor.author	Casals i Farré, Núria
dc.contributor.author	Prósper, Felipe
dc.contributor.author	Ferrés Padró, Eduard
dc.contributor.author	Giner Tarrida, Lluís
dc.date	2019-09-19T15:05:06Z
dc.date	2019-09-19T15:05:06Z
dc.date	2012-09-05
dc.identifier	Atari, Maher; Gil Recio, Carlos; Fabregat, Marc; García Fernández, Daniel; Barajas, Miguel; Carrasco García, Miguel Ángel; Jung, Han-Sung; Hernández Alfaro, Federico; Casals Farré, Núria; Prósper, Felipe; Ferrés Padró, Eduard; Giner Tarrida, Lluís. «Dental pulp of the third molar: a new source of pluripotent-like stem cells». Journal of Cell Science, 2012, vol. 125, núm. 14, p. 3343–3356. Disponible en: <https://jcs.biologists.org/content/125/14/3343>. Fecha de acceso: 19 sept. 2019. http://dx.doi.org/10.1242/jcs.096537
dc.identifier	0021-9533
dc.identifier	http://dx.doi.org/10.1242/jcs.096537
dc.identifier.uri	http://hdl.handle.net/20.500.12328/1234
dc.description	Dental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. These cells are SSEA4+, OCT3/4+, NANOG+, SOX2+, LIN28+, CD13+, CD105+, CD342, CD452, CD90+, CD29+, CD73+, STRO1+ and CD1462, and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.
dc.description	postprint
dc.format	14-24
dc.format	application/pdf
dc.format	application/pdf
dc.language	eng
dc.publisher	Company of Biologists
dc.relation	Journal of Cell Science
dc.relation	125;14
dc.rights	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Polpa dental
dc.subject	Dental pulp
dc.subject	Pulpa dental
dc.subject	Cèl·lules mare
dc.subject	Stem cells
dc.subject	Células madre
dc.subject	DPPSC
dc.subject	CGH technique
dc.subject	616.3
dc.title	Dental pulp of the third molar: a new source of pluripotent-like stem cells
dc.type	info:eu-repo/semantics/article

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