dc.contributor.author |
Gruzieva, Olena |
dc.contributor.author |
Antó i Boqué, Josep Maria |
dc.contributor.author |
Bustamante Pineda, Mariona |
dc.contributor.author |
Guxens, Mònica |
dc.contributor.author |
Sunyer Deu, Jordi |
dc.contributor.author |
Melén, Erik |
dc.date |
2017 |
dc.identifier.citation |
Gruzieva O, Xu CJ, Breton CV, Annesi-Maesano I, Antó JM, Auffray C. et al. Epigenome-Wide Meta-Analysis of Methylation in Children Related to Prenatal NO2 Air Pollution Exposure. Environ Health Perspect. 2017 Jan;125(1):104-110. doi: 10.1289/EHP36 |
dc.identifier.citation |
0091-6765 |
dc.identifier.citation |
http://dx.doi.org/10.1289/EHP36 |
dc.identifier.uri |
http://hdl.handle.net/10230/28166 |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Environmental health perspectives |
dc.rights |
Reproduced with permission from Environmental Health Perspectives |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Contaminació de l'aire |
dc.subject |
Embaràs |
dc.subject |
Anomalies congènites |
dc.title |
Epigenome-Wide Meta-Analysis of Methylation in Children Related to Prenatal NO2 Air Pollution Exposure |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |
dc.description.abstract |
BACKGROUND: Prenatal exposure to air pollution is considered to be associated with adverse effects on child health. This may partly be mediated by mechanisms related to DNA methylation. OBJECTIVES: We investigated associations between exposure to air pollution, using nitrogen dioxide (NO2) as marker, and epigenome-wide cord blood DNA methylation. METHODS: We meta-analyzed the associations between NO2 exposure at residential addresses during pregnancy and cord blood DNA methylation (Illumina 450K) in four European and North American studies (n = 1,508) with subsequent look-up analyses in children ages 4 (n = 733) and 8 (n = 786) years. Additionally, we applied a literature-based candidate approach for antioxidant and anti-inflammatory genes. To assess influence of exposure at the transcriptomics level, we related mRNA expression in blood cells to NO2 exposure in 4- (n = 111) and 16-year-olds (n = 239). RESULTS: We found epigenome-wide significant associations [false discovery rate (FDR) p < 0.05] between maternal NO2 exposure during pregnancy and DNA methylation in newborns for 3 CpG sites in mitochondria-related genes: cg12283362 (LONP1), cg24172570 (3.8 kbp upstream of HIBADH), and cg08973675 (SLC25A28). The associations with cg08973675 methylation were also significant in the older children. Further analysis of antioxidant and anti-inflammatory genes revealed differentially methylated CpGs in CAT and TPO in newborns (FDR p < 0.05). NO2 exposure at the time of biosampling in childhood had a significant impact on CAT and TPO expression. CONCLUSIONS: NO2 exposure during pregnancy was associated with differential offspring DNA methylation in mitochondria-related genes. Exposure to NO2 was also linked to differential methylation as well as expression of genes involved in antioxidant defense pathways |