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Title:	LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication
Author:	Galão, Rui Pedro Ribeiro; Chari, Ashwin; Alves-Rodrigues, Isabel; Lobão, Daniela; Mas, Antonio; Kambach, Christian; Fischer, Utz; Díez Antón, Juana, 1962-
Abstract:	LSm1-7 complexes promote cellular mRNA degradation, in addition to translation and replication of positive-strand RNA viruses such as the Brome mosaic virus (BMV). Yet, how LSm1-7 complexes act on their targets remains elusive. Here, we report that reconstituted recombinant LSm1-7 complexes directly bind to two distinct RNA-target sequences in the BMV genome, a tRNA-like structure at the 3'-untranslated region and two internal A-rich single-stranded regions. Importantly, in vivo analysis shows that these sequences regulate the translation and replication of the BMV genome. Furthermore, both RNA-target sequences resemble those found for Hfq, the LSm counterpart in bacteria, suggesting conservation through evolution. Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions.
Abstract:	This work was supported by grants from the Spanish Ministerio de Educación y Ciencia (BFU2007-66933/BMC) and the German Research Foundation (DFG-FOR855). I.A.-R. and D.L. were supported by Fundaçao para a Ciência e Tecnología (SARH/BD/9630/2002; SFRH/BD/37047/2007)
Subject(s):	-Interaccions RNA-proteïna -Saccharomyces cerevisiae -- Metabolisme -BMV -LSm1-7 -RNA virus -Sm proteins -Translation -Replication
Rights:	Copyright © 2010, RNA by RNA Society. Published version available at http://rnajournal.cshlp.org/content/16/4/817.long
Document type:	Article Article - Published version
Published by:	Cold Spring Harbor Laboratory Press (CSHL Press)
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