Título:
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Validated methodology for quantifying infestation levels of dreissenid mussels in environmental DNA (eDNA) samples
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Autor/a:
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Peñarrubia Lozano, Luis; Alcaraz Cazorla, Carles; Vaate, Abraham bij de; Sanz Ball-llosera, Núria; Pla Zanuy, Carles; Vidal Fàbrega, Oriol; Viñas de Puig, Jordi
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Otros autores:
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Ministerio de Ciencia e Innovación (Espanya) |
Abstract:
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The zebra mussel (Dreissena polymorpha Pallas, 1771) and the quagga mussel (D. rostriformis Deshayes, 1838) are successful invasive bivalves with substantial ecological and economic impacts in freshwater systems once they become established. Since their eradication is extremely difficult, their detection at an early stage is crucial to prevent spread. In this study, we optimized and validated a qPCR detection method based on the histone H2B gene to quantify combined infestation levels of zebra and quagga mussels in environmental DNA samples. Our results show specific dreissenid DNA present in filtered water samples for which microscopic diagnostic identification for larvae failed. Monitoring a large number of locations for invasive dreissenid species based on a highly specific environmental DNA qPCR assay may prove to be an essential tool for management and control plans focused on prevention of establishment of dreissenid mussels in new locations |
Abstract:
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We are indebted to different colleagues for zebra mussel sample collections: Concha Durán (Ebro Hydrografic
Confederation, Spain) for the logistic advice; Anna Terrats and Carolina Solà (Catalan Water Agency from the
Government of Catalonia, Spain) for the filtered-water environmental samples. We would thank to Mariona
Palacios for her collaboration in the analysis into her Final Master Project. This research was carried out
within the objectives of the research project CGL200909407 of the Spanish Ministerio de Ciencia e Innovación
(MICINN) |
Materia(s):
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-Genètica de poblacions -Population genetics -Musclos d’aigua dolça -- Genètica -Freshwater mussels -- Genetics -Animals invasors -Invasions biològiques -Introduced organisms -Biological invasions |
Derechos:
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Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/ |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Nature Publishing Group
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