Título:
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A nuclear-directed human pancreatic ribonuclease (PE5) targets the metabolic phenotype of cancer cells
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Autor/a:
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Vert Company, Anna; Castro Gallegos, Jessica; Ribó i Panosa, Marc; Benito i Mundet, Antoni; Vilanova i Brugués, Maria
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Otros autores:
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Ministerio de Ciencia e Innovación (Espanya); Ministerio de Economía y Competitividad (Espanya) |
Abstract:
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Ribonucleases represent a new class of antitumor RNA-damaging drugs.
However, many wild-type members of the vertebrate secreted ribonuclease family are
not cytotoxic because they are not able to evade the cytosolic ribonuclease inhibitor.
We previously engineered the human pancreatic ribonuclease to direct it to the cell
nucleus where the inhibitor is not present. The best characterized variant is PE5 that
kills cancer cells through apoptosis mediated by the p21WAF1/CIP1 induction and the
inactivation of JNK. Here, we have used microarray-derived transcriptional profiling
to identify PE5 regulated genes on the NCI/ADR-RES ovarian cancer cell line. RT-qPCR
analyses have confirmed the expression microarray findings. The results show that
PE5 cause pleiotropic effects. Among them, it is remarkable the down-regulation of
multiple genes that code for enzymes involved in deregulated metabolic pathways
in cancer cells |
Abstract:
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This work has been supported by grants BFU2009-06935 and BIO2013-43517 from MINECO (Spain) and SING12/0 from UdG (Spain). |
Materia(s):
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-Càncer -- Tractament -Cancer -- Treatment -Ribonucleases -Cèl·lules canceroses -Cancer cells |
Derechos:
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Attribution 3.0 Spain
http://creativecommons.org/licenses/by/3.0/es/ |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Impact Journals
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Compartir:
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