dc.contributor.author |
Vara, Covadonga |
dc.contributor.author |
Paytuví Gallart, Andreu |
dc.contributor.author |
Cuartero, Yasmina |
dc.contributor.author |
Le Dily, François |
dc.contributor.author |
Garcia, Francisca |
dc.contributor.author |
Salvà-Castro, Judit |
dc.contributor.author |
Gómez-H, Laura |
dc.contributor.author |
Julià, Eva |
dc.contributor.author |
Moutinho, Catia |
dc.contributor.author |
Aiese Cigliano, Riccardo |
dc.contributor.author |
Sanseverino, Walter |
dc.contributor.author |
Fornas Carreño, Oscar |
dc.contributor.author |
Pendás, Alberto M. |
dc.contributor.author |
Heyn, Holger |
dc.contributor.author |
Waters, Paul D. |
dc.contributor.author |
Marti-Renom, Marc A. |
dc.contributor.author |
Ruiz Herrera, Aurora |
dc.date |
2019 |
dc.identifier.citation |
Vara C, Paytuví-Gallart A, Cuartero Y, Le Dily F, Garcia F, Salvà-Castro J, Gómez-H L, Julià E, Moutinho C, Aiese Cigliano R, Sanseverino W, Fornas O, Pendás AM, Heyn H, Waters PD, Marti-Renom MA, Ruiz-Herrera A. Three-dimensional genomic structure and cohesin occupancy correlate with transcriptional activity during spermatogenesis. Cell Rep. 2019; 28(2):352-367.e9. DOI 10.1016/j.celrep.2019.06.037 |
dc.identifier.citation |
2211-1247 |
dc.identifier.citation |
http://dx.doi.org/10.1016/j.celrep.2019.06.037 |
dc.identifier.uri |
http://hdl.handle.net/10230/42347 |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Elsevier |
dc.relation |
Cell Rep. 2019; 28(2):352-367.e9 |
dc.relation |
info:eu-repo/grantAgreement/EC/FP7/609989 |
dc.relation |
info:eu-repo/grantAgreement/ES/2PE/BFU2017-89408-R |
dc.relation |
info:eu-repo/grantAgreement/ES/1PE/BFU2013-47736-P |
dc.relation |
info:eu-repo/grantAgreement/ES/2PE/BFU2017-85926-P |
dc.relation |
info:eu-repo/grantAgreement/ES/1PE/CGL2014-54317-P |
dc.relation |
info:eu-repo/grantAgreement/ES/2PE/CGL2017-83802-P |
dc.relation |
info:eu-repo/grantAgreement/ES/1PE/BES-2015-072924 |
dc.relation |
info:eu-repo/grantAgreement/ES/2PE/SAF2017-89109-P |
dc.relation |
info:eu-repo/grantAgreement/EC/H2020/676556 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
© 2019 The Author(s).This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
dc.rights |
http://creativecommons.org/licenses/by/4.0/ |
dc.title |
Three-dimensional genomic structure and cohesin occupancy correlate with transcriptional activity during spermatogenesis |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Mammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining in situ chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) of CCCTC-binding factor (CTCF) and meiotic cohesins, coupled with confocal and super-resolution microscopy. Spermatogonia presents well-defined compartment patterns and topological domains. However, chromosome occupancy and compartmentalization are highly re-arranged during prophase I, with cohesins bound to active promoters in DNA loops out of the chromosomal axes. Compartment patterns re-emerge in round spermatids, where cohesin occupancy correlates with transcriptional activity of key developmental genes. The compact sperm genome contains compartments with actively transcribed genes but no fine-scale topological domains, concomitant with the presence of protamines. Overall, we demonstrate how genome-wide cohesin occupancy and transcriptional activity is associated with three-dimensional (3D) remodeling during spermatogenesis, ultimately reprogramming the genome for the next generation. |
dc.description.abstract |
This work was supported by the Ministry of Economy and Competitiveness (BFU2017-89408-R to A.M.P.; BFU2013-47736-P and BFU2017-85926-P to M.A.M.-R.; and CGL2014-54317-P and CGL2017-83802-P to A.R.-H.) and the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR) (DI2015 to A.R.-H. and R.A.C., as well as SGR468 to M.A.M-R.). Work at CRG, BIST, and UPF was in part funded by the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’ (SEV-2012-0208), and ‘Centro de Excelencia María de Maeztu 2016-2019.’ CIC-IBMCC is supported by the Programa de Apoyo a Planes Estratégicos de Investigación de Estructuras de Investigación de Excelencia, co-funded by Junta de Castilla y León (CSI239P18) and the European Regional Development Fund (CLC–2017–01). C.V. is supported by a FPI predoctoral fellowship from the Ministry of Economy and Competitiveness (BES-2015-072924). A.P.-G. is supported by a ‘Doctorats Industrials’ predoctoral fellowship (AGAUR). H.H. is a Miguel Servet (CP14/00229) researcher funded by the Spanish Institute of Health Carlos III (ISCIII), the Agencia Estatal de Investigación (AEI), and FEDER (SAF2017-89109-P). C.M. is an Asociación Española Contra el Cáncer (AECC) postdoctoral fellow. M.A.M.-R. acknowledges support by the European Research Council under the 7th Framework Program FP7/2007-2013 (ERC grant agreement 609989) and the European Union’s Horizon 2020 Research and Innovation Programme (grant agreement 676556). A.M.P. and A.R.-H. also acknowledge support from MeioNet (BFU2015-71786-REDT). |