Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity

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dc.contributor.author	Carmona, Mercè
dc.contributor.author	de Cubas, Laura
dc.contributor.author	Bautista, Eric
dc.contributor.author	Moral-Blanch, Marta
dc.contributor.author	Medrano-Fernández, Iria
dc.contributor.author	Sitia, Roberto
dc.contributor.author	Boronat i Llop, Susanna, 1965-
dc.contributor.author	Ayté del Olmo, José
dc.contributor.author	Hidalgo Hernando, Elena
dc.date	2019
dc.identifier.citation	Carmona M, de Cubas L, Bautista E, Moral-Blanch M, Medraño-Fernández I, Sitia R, Boronat S, Ayté J, Hidalgo E. Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity. Nat Commun. 2019; 10(1):4526. DOI 10.1038/s41467-019-12475-0
dc.identifier.citation	2041-1723
dc.identifier.citation	http://dx.doi.org/10.1038/s41467-019-12475-0
dc.identifier.uri	http://hdl.handle.net/10230/42405
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Nature Research
dc.relation	Nat Commun. 2019; 10(1):4526
dc.relation	info:eu-repo/grantAgreement/ES/1PE/BFU2015-68350-P
dc.relation	info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights	http://creativecommons.org/licenses/by/4.0/
dc.subject	Chemical tools
dc.subject	Stress signalling
dc.title	Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.abstract	Genetically encoded probes monitoring H2O2 fluctuations in living organisms are key to decipher redox signaling events. Here we use a new probe, roGFP2-Tpx1.C169S, to monitor pre-toxic fluctuations of peroxides in fission yeast, where the concentrations linked to signaling or to toxicity have been established. This probe is able to detect nanomolar fluctuations of intracellular H2O2 caused by extracellular peroxides; expression of human aquaporin 8 channels H2O2 entry into fission yeast decreasing membrane gradients. The probe also detects H2O2 bursts from mitochondria after addition of electron transport chain inhibitors, the extent of probe oxidation being proportional to the mitochondrial activity. The oxidation of this probe is an indicator of steady-state levels of H2O2 in different genetic backgrounds. Metabolic reprogramming during growth in low-glucose media causes probe reduction due to the activation of antioxidant cascades. We demonstrate how peroxiredoxin-based probes can be used to monitor physiological H2O2 fluctuations.
dc.description.abstract	This work is supported by the Ministerio de Ciencia, Innovación y Universidades (Spain), PLAN E and FEDER (BFU2015-68350-P and PGC2018-093920-B-I00 to E.H.) and by Unidad de Excelencia María de Maeztu (MDM-2014-0370). The Oxidative Stress and Cell Cycle group is also supported by Generalitat de Catalunya (Spain) (2017-SGR-539). E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain).

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