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Título:	Carnitine palmitoyltransferase 1 increases lipolysis, UCP1 protein expression and mitochondrial activity in brown adipocytes
Autor/a:	Calderón Domínguez, María; Sebastián Muñoz, David; Fucho Salvador, Raquel; Weber Blattes, Minéia; Mir Bonnín, Joan Francesc; García-Casarrubios, Ester; Obregón, Maria Jesús; Zorzano Olarte, Antonio; Valverde, Ángela M.; Serra i Cucurull, Dolors; Herrero Rodríguez, Laura
Otros autores:	Universitat de Barcelona
Abstract:	The discovery of active brown adipose tissue (BAT) in adult humans and the fact that it is reduced in obese and diabetic patients have put a spotlight on this tissue as a key player in obesity-induced metabolic disorders. BAT regulates energy expenditure through thermogenesis; therefore, harnessing its thermogenic fat-burning power is an attractive therapeutic approach. We aimed to enhance BAT thermogenesis by increasing its fatty acid oxidation (FAO) rate. Thus, we expressed carnitine palmitoyltransferase 1AM (CPT1AM), a permanently active mutant form of CPT1A (the rate-limiting enzyme in FAO), in a rat brown adipocyte (rBA) cell line through adenoviral infection. We found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements. This indicates that CPT1AM-mediated increase in brown adipocytes FAO may be a new approach to the treatment of obesity-induced disorders.
Materia(s):	-Carnitina palmitoïl-transferasa 1 -Teixit adipós -Obesitat -Carnitine palmitoyltransferase I -Adipose tissues -Obesity
Derechos:	cc-by (c) Calderon-Dominguez, María et al., 2016 http://creativecommons.org/licenses/by/3.0/es
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	Public Library of Science (PLoS)
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