Application of an innovative water-assisted ultraviolet C light technology for the inactivation of microorganisms in tomato processing industries 

    Abadias, Maribel; Colás-Medà, Pilar; Viñas, Inmaculada; Bobo, Gloria; Aguiló-Aguayo, Ingrid (2020-08-30)

    We aimed to study the efficacy of a water-assisted UVC light device (WUVC) as an innovative clean technology for the disinfection of fresh sound tomatoes and processing wash water and water turbidity was evaluated as a ...

    Copy Number Variation on ABCC2-DNMBP Loci Affects the Diversity and Composition of the Fecal Microbiota in Pigs 

    Ramayo-Caldas, Yuliaxis; Crespo-Piazuelo, Daniel; Morata, Jordi; González-Rodríguez, Olga; Sebastià, Cristina; Castello, Anna; Dalmau, Antoni; Ramos-Onsins, Sebastian; Alexiou, Konstantinos G.; Folch, Josep M.; Quintanilla, Raquel; Ballester, Maria (2023-05-31)

    Genetic variation in the pig genome partially modulates the composition of porcine gut microbial communities. Previous studies have been focused on the association between single nucleotide polymorphisms (SNPs) and the gut ...

    Design strategies for positively charged endolysins: Insights into Artilysin development 

    Carratalá, Jose Vicente; Alomar, Oscar; Garcia-Fruitos, Elena; Ferrer-Miralles, Neus (2023-09-06)

    Endolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. The use of genetic ...

    Exploring the impact of the recombinant Escherichia coli strain on defensins antimicrobial activity: BL21 versus Origami strain 

    López-Cano, Adrià; Martínez-Miguel, Marc; Guasch, Judith; Ratera, Imma; Alomar, Oscar; Garcia-Fruitos, Elena (2022-05-09)

    The growing emergence of microorganisms resistant to antibiotics has prompted the development of alternative antimicrobial therapies. Among them, the antimicrobial peptides produced by innate immunity, which are also known ...

    Exploring the therapeutic potential of recombinant bovine β-defensins for antimicrobial and anti-inflammatory functions in sepsis management 

    Saubi, Cristina; Baltà-Foix, Ricardo; Carratalá, Jose Vicente; Fàbregas, Francesc; Sandín, Daniel; Torrent, Marc; Garcia-Fruitos, Elena; Arís, Anna (2025-09-02)

    β-defensins are multifunctional peptides of the host immune system involved in responses to infectious diseases. We investigated the potential of five recombinant proteins based on bovine β-defensins (bovine neutrophil ...

    Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications 

    Roca-Pinilla, Ramon; Fortuna, Sara; Natalello, Antonino; Sánchez-Chardi, Alejandro; Ami, Diletta; Alomar, Oscar; Garcia-Fruitos, Elena (2020-09-04)

    Background Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior. Because of their nature, IBs have been explored to be used as ...

    Feeding pasteurized waste milk to preweaned dairy calves changes fecal and upper respiratory tract microbiota 

    Maynou, Georgina; Chester-Jones, Hugh; Bach, Alex; Terré, Marta (2019-06-06)

    In the present study bacterial communities from both, the gastrointestinal and respiratory tract of pre-weaned dairy calves fed two different milk-feeding programs were characterized using 16S rRNA gene sequencing. Twenty ...

    Grupo de Microbiología Ambiental y Sostenibilidad del IRTA 

    Viñas, Marc; Prenafeta-Boldú, Francesc Xavier; Guivernau, Miriam; Buron-Moles, Gemma; Fernández García, Belén; Nogales, Amaia; Biel, Carmen (2024-12)

    Gut barrier-microbiota imbalances in early life lead to higher sensitivity to inflammation in a murine model of C-section delivery 

    Barone, M.; Ramayo-Caldas, Yuliaxis; Estellé, J.; Tambosco, K.; Chadi, S.; Maillard, F.; Gallopin, M.; Planchais, J.; Chain, F.; Kropp, C.; Rios-Covian, D.; Sokol, H.; Brigidi, P.; Langella, P.; Martín, R. (2023-07-03)

    Background Most interactions between the host and its microbiota occur at the gut barrier, and primary colonizers are essential in the gut barrier maturation in the early life. The mother–ofspring transmission of ...

    Gut eukaryotic communities in pigs: diversity, composition and host genetics contribution 

    Ramayo-Caldas, Yuliaxis; Prenafeta-Boldú, Francesc; Zingaretti, Laura M.; Gonzalez-Rodriguez, Olga; Dalmau, Antoni; Quintanilla, Raquel; Ballester, Maria (2020-05-07)

    Background The pig gut microbiome harbors thousands of species of archaea, bacteria, viruses and eukaryotes such as protists and fungi. However, since the majority of published studies have been focused on prokaryotes, ...

    Lactiplantibacillus plantarum: a new example of inclusion body producing bacteria 

    Baltà, Ricardo; Serrano-Adrover, Caterina; López-Cano, Adrià; Gifre-Renom, Laia; Sanchez-Chardi, Alejandro; Alomar, Oscar; Garcia-Fruitos, Elena (2023-06-09)

    Background Lactic Acid Bacteria such as Lactococcus lactis, Latilactobacillus sakei (basonym: Lactobacillus sakei) and Lactiplantibacillus plantarum (basonym: Lactobacillus plantarum) have gained importance as recombinant ...

    LysJEP8: A promising novel endolysin for combating multidrug-resistant Gram-negative bacteria 

    Carratalá, Jose Vicente; Ferrer‐Miralles, Neus; Garcia-Fruitos, Elena; Alomar, Oscar (2024-06-12)

    Antimicrobial resistance (AMR) is an escalating global health crisis, driven by the overuse and misuse of antibiotics. Multidrug-resistant Gram-negative bacteria, such as Pseudomonas aeruginosa, Acinetobacter baumannii, ...

    Nano zerovalent iron boosts methane content in biogas and reshapes microbial communities in long-term anaerobic digestion of pig slurry 

    Cerrillo, Míriam; Guivernau, Miriam; Burgos Rodríguez, Laura; Riau, Victor; Bonmati, August (2024-12-09)

    Adding nanoscale zero-valent iron (nZVI) to the anaerobic digestion (AD) process for livestock manure has been shown to significantly enhance methane content by influencing microbial communities and metabolic pathways. ...

    Optimizing Hydrogen Production Through Efficient Organic Matter Oxidation Performed by Microbial Electrolysis Cells 

    Marchetti, Angela; Cerrillo, Míriam; Lauri, Roberto; Zeppilli, Marco (2025-04-18)

    Microbial electrolysis cells (MECs) represent a pioneering technology for sustainable hydrogen production by leveraging bioelectrochemical processes. This study investigates the performance of a single-chamber cathodic ...

    Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies 

    López-Cano, Adrià; Sicilia, Paula; Gaja, Clara; Alomar, Oscar; Garcia-Fruitos, Elena (2022-09-20)

    Recombinant protein production in bacteria is often accompanied by the formation of aggregates, known as inclusion bodies (IBs). Although several strategies have been developed to minimize protein aggregation, many ...

    Role of mycorrhizas and root exudates in plant uptake of soil nutrients (calcium, iron, magnesium, and potassium): has the puzzle been completely solved? 

    Sardans, Jordi; Lambers, Hans; Preece, Catherine; Alrefaei, Abdulwahed Fahad; Penuelas, Josep (2023-03-14)

    Anthropogenic global change is driving an increase in the frequency and intensity of drought and flood events, along with associated imbalances and limitation of several soil nutrients. In the context of an increasing human ...

    Startup strategy for nitrogen removal via nitrite in a BAF system 

    Gabarró, Jordi; Guivernau, Miriam; Burgos, Laura; Garanto, Oswald; Bonmatí, August (2018-10-26)

    A biological aerated filter (BAF) pilot plant consisting of two reactors (aerobic and anoxic one) was used to determine a strategy to remove nitrogen via nitrite. RNA/DNA analysis was performed to assess microbial activity ...

    The future of recombinant host defense peptides 

    Roca-Pinilla, Ramon; Lisowski, Leszek; Alomar, Oscar; Garcia-Fruitos, Elena (2022-12-21)

    The antimicrobial resistance crisis calls for the discovery and production of new antimicrobials. Host defense peptides (HDPs) are small proteins with potent antibacterial and immunomodulatory activities that are attractive ...

    Time to consider ruling out inclusion bodies denaturing protocols for spontaneous solubilization of biologically active proteins 

    Baltà, Ricardo; Garcia-Fruitos, Elena; Arís, Anna (2024-10-30)

    The formation of inclusion bodies (IBs) in microbial cell factories is a very common process occurring during recombinant protein production. Different protocols have been developed for the extraction of soluble proteins ...