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dc.contributor.author | Moisés, Jorge |
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dc.contributor.author | Navarro Ponz, Alfons |
dc.contributor.author | Santasusagna, Sandra |
dc.contributor.author | Viñolas Segarra, Núria |
dc.contributor.author | Molins López-Rodó, Laureano |
dc.contributor.author | Ramirez, José |
dc.contributor.author | Osorio, Jeisson |
dc.contributor.author | Saco, Adela |
dc.contributor.author | Castellano, Joan Josep |
dc.contributor.author | Muñoz García, Carmen |
dc.contributor.author | Morales, Sara |
dc.contributor.author | Monzó Planella, Mariano |
dc.contributor.author | Marrades Sicart, Ramon Ma. |
dc.date | 2018-06-12T10:59:36Z |
dc.date | 2018-06-12T10:59:36Z |
dc.date | 2017-12-13 |
dc.date | 2018-06-12T10:59:36Z |
dc.identifier | 1471-2466 |
dc.identifier | 674898 |
dc.identifier | 29237428 |
dc.identifier.uri | http://hdl.handle.net/2445/122905 |
dc.description | BACKGROUND: NKX2-1, a key molecule in lung development, is highly expressed in non-small cell lung cancer (NSCLC), particularly in lung adenocarcinoma (ADK), where it is a diagnostic marker. Studies of the prognostic role of NKX2-1 in NSCLC have reported contradictory findings. Two microRNAs (miRNAs) have been associated with NKX2-1: miR-365, which targets NKX2-1; and miR-33a, which is downstream of NKX2-1. We have examined the effect of NKX2-1, miR-365 and miR-33a on survival in a cohort of early-stage NSCLC patients and in sub-groups of patients classified according to the mutational status of TP53, KRAS, and EGFR. METHODS: mRNA and miRNA expression was determined using TaqMan assays in 110 early-stage NSCLC patients. TP53, KRAS, and EGFR mutations were assessed by Sanger sequencing. RESULTS: NKX2-1 expression was upregulated in never-smokers (P = 0.017), ADK (P < 0.0001) and patients with wild-type TP53 (P = 0.001). A negative correlation between NKX2-1 and miR-365 expression was found (ρ = -0.287; P = 0.003) but there was no correlation between NKX2-1 and miR-33a expression. Overall survival (OS) was longer in patients with high expression of NKX2-1 than in those with low expression (80.8 vs 61.2 months (P = 0.035), while a trend towards longer OS was observed in patients with low miR-365 levels (P = 0.07). The impact of NKX2-1 on OS and DFS was higher in patients with neither TP53 nor KRAS mutations. Higher expression of NKX2-1 was related to higher OS (77.6 vs 54 months; P = 0.017) and DFS (74.6 vs 57.7 months; P = 0.006) compared to low expression. The association between NKX2-1 and OS and DFS was strengthened when the analysis was limited to patients with stage I disease (P = 0.005 and P=0.003 respectively). CONCLUSIONS: NKX2-1 expression impacts prognosis in early-stage NSCLC patients, particularly in those with neither TP53 nor KRAS mutations. |
dc.format | 9 p. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | BioMed Central |
dc.relation | Reproducció del document publicat a: https://doi.org/10.1186/s12890-017-0542-z |
dc.relation | BMC Pulmonary Medicine, 2017, vol. 17, num. 197 |
dc.relation | https://doi.org/10.1186/s12890-017-0542-z |
dc.rights | cc-by (c) Moisés, Jorge et al., 2017 |
dc.rights | http://creativecommons.org/licenses/by/3.0/es |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Càncer de pulmó |
dc.subject | Marcadors tumorals |
dc.subject | Lung cancer |
dc.subject | Tumor markers |
dc.title | NKX2-1 expression as a prognostic marker in early-stage non-small-cell lung cancer |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |