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Títol:	Directed differentiation of human pluripotent stem sells for the generation of high-order kidney organoids
Autor/a:	Selfa, Idoia Lucía; Gallo, Maria; Montserrat, Nuria; Garreta, Elena
Notes:	Our understanding in the inherent properties of human pluripotent stem cells (hPSCs) have made possible the development of differentiation procedures to generate three-dimensional tissue-like cultures, so-called organoids. Here we detail a stepwise methodology to generate kidney organoids from hPSCs. This is achieved through direct differentiation of hPSCs in two-dimensional monolayer culture toward the posterior primitive streak fate, followed by induction of intermediate mesoderm-committed cells, which are further aggregated and cultured in three-dimensions to generate kidney organoids containing segmented nephron-like structures in a process that lasts 20 days. We also provide a concise description on how to assess renal commitment during the time course of kidney organoid generation. This includes the use of flow cytometry and immunocytochemistry analyses for the detection of specific renal differentiation markers.
Matèries:	-Ronyó -Cèl·lules mare -Diferenciació cel·lular -Kidney -Stem cells -Cell diferentiation
Drets:	(c) Springer Nature, 2020 http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/embargoedAccess
Tipus de document:	Capítol o part de llibre Article - Versió acceptada
Publicat per:	Springer Nature
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