dc.contributor
Ministerio de Ciencia e Innovación (Espanya)
dc.contributor.author
Serrano, Soraya
dc.contributor.author
Callís Figueres, Mariona
dc.contributor.author
Vilanova i Brugués, Maria
dc.contributor.author
Benito i Mundet, Antoni
dc.contributor.author
Laurents, Douglas V.
dc.contributor.author
Ribó i Panosa, Marc
dc.contributor.author
Bruix, Marta
dc.date.issued
info:eu-repo/date/embargoEnd/2026-01-01
dc.identifier
http://hdl.handle.net/10256/11967
dc.description.abstract
Onconase® FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase® sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase's® active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure-function relationships, we report here the nearly complete NMR 1H, 13C and 15N resonance chemical shift assignments at pH 5.2 and 35 C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase ® is conserved in the FL-G zymogen
dc.description.abstract
This work was supported by the projects, CTQ2008-0080, CTQ2010-21567-C02-02 and BFU2009-06935/ BMC from MICINN and PUG2008A from the Universitat de Girona
dc.format
application/pdf
dc.relation
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12104-012-9367-0
dc.relation
info:eu-repo/semantics/altIdentifier/issn/1874-2718
dc.relation
info:eu-repo/semantics/altIdentifier/eissn/1874-270X
dc.relation
info:eu-repo/grantAgreement/MICINN//BFU2009-06935/ES/Bases Moleculares Del Plegamiento Y Citotoxicidad De Las Ribonucleasas Pancreaticas. Evaluacion De La Actividad Citotoxica Y Diseño De Estrategias Para Su Control Mediante Splicing Proteico./
dc.rights
Tots els drets reservats
dc.rights
info:eu-repo/semantics/embargoedAccess
dc.source
© Biomolecular NMR Assignments, 2013, vol. 7, p. 13-15
dc.source
Articles publicats (D-B)
dc.subject
Enginyeria de proteïnes
dc.subject
Protein engineering
dc.title
1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
