Insight into a Successful Development of Biocontrol Agents: Production, Formulation, Packaging, and Shelf Life as Key Aspects

Abstract

Biocontrol agents (BCAs) have been proposed and studied over recent decades as a suita‐ ble alternative to diminish or substitute synthetic fungicides used to control pre‐ and postharvest diseases. However, the development of BCAs has many constraints and obstacles that would have to be overcome before they could be successfully implemented in the market. For the BCA commer‐ cial development, the microorganism should be mass‐produced on a large‐scale, and, inde‐ pendently of the method used for the production, a particular plan regarding the formulation of BCAs by multidisciplinary approaches (liquid or solid) is required to optimize the yield, efficacy, and shelf life of the developed product. Unfortunately, not all BCAs can survive the conditions im‐ posed during the formulation process. Improved stability can be achieved by either using special conditions during growing or by adding protective substances to the formulation medium. Finally, BCAs should be formulated in such a way as to guarantee long‐term stability and ease of application of the product. Therefore, an accurate range of the packaging conditions should be considered to extend the shelf life of the formulated product, preferably up to two years. Herein, we discussed the main aspects regarding the production, formulation, packaging, and shelf life of BCAs.