Ciències Fisiològiques: Enviaments recents
Ara mostrant els elements 141-160 de 826
Microglial cells in astroglial cultures: a cautionary note
Primary rodent astroglial-enriched cultures are the most popular model to study astroglial biology in vitro. From the original methods described in the 1970's a great number of minor modifications have been incorporated ...
VEGF-A/Notch-induced podosomes proteolyse basement membrane collagen-IV during retinal sprouting angiogenesis
During angiogenic sprouting, endothelial tip cells emerge from existing vessels in a process that requires vascular basement membrane degradation. Here, we show that F-actin/cortactin/P-Src-based matrix-degrading microdomains ...
TIGAR (chromosome 12 open reading frame 5)
Human TIGAR protein consists of 270 arninoacids, with a molecular weight of 30063 Da. It is cornposed of a bisphosphatase active site in which two histidines, His-11 and His-198, and one glutamic acid, Glu-1 02, are key ...
Acute mountain sickness susceptibility and basic cognitive function after a brief simulated altitude of 4800 m.
Twelve climbers with not been exposed in the last 12 months at high altitude were evaluated using verbal, spatial, reasoning and numerical tasks from Thurstone's (1969) Primary Mental Abilities (PMA) test. These tasks were ...
Dynamic epigenetic age mosaicism in the human atherosclerotic artery
Accelerated epigenetic ageing, a promising marker of disease risk, has been detected in peripheral blood cells of atherosclerotic patients, but evidence in the vascular wall is lacking. Understanding the trends of epigenetic ...
Mutation analysis in cystic fibrosis
The article by Lemna et al. (Feb. 1 issue)1 furthers the evaluation of the ΔF508 mutation, which is associated with some cases of cystic fibrosis. Although its real effect may be to help in documenting the substantial ...
Scrfl restriction fragment length polymorphism at the D7S23 locus (probe pKM.19), closely linked to cystic fibrosis
Source/Description: pKM.19 is a 1.0 kb EcoRI genomic fragment in pUC13 (ref. 1,2). pPl was isolated independently but contains the same fragment as pKM.19 (ref. 3)...
Integrin-linked kinase stabilizes myotendinous junctions and protects muscle from stress-induced damage.
Skeletal muscle expresses high levels of integrin-linked kinase (ILK), predominantly at myotendinous junctions (MTJs) and costameres. ILK binds the cytoplasmic domain of beta1 integrin and mediates phosphorylation of protein ...
Diazoxide attenuates autoimmune encephalomyelitis and modulates lymphocyte proliferation and dendritic cell functionality
Activation of mitochondrial ATP-sensitive potassium (KATP) channels is postulated as an effective mechanism to confer cardio and neuroprotection, especially in situations associated to oxidative stress. Pharmacological ...
Epithelial-to-mesenchymal transition mediates docetaxel resistance and high risk of relapse in prostate cancer
Molecular characterization of radical prostatectomy specimens after systemic therapy may identify a gene expression profile for resistance to therapy. This study assessed tumor cells from patients with prostate cancer ...
Genome-Wide DNA Methylation Analysis Identifies Novel Hypomethylated Non-Pericentromeric Genes with Potential Clinical Implications in ICF Syndrome
Introduction and Results Immunodeficiency, centromeric instability and facial anomalies syndrome (ICF) is a rare autosomal recessive disease, characterized by severe hypomethylation in pericentromeric regions of chromosomes ...
BRCA1 CpG island hypermethylation predicts sensitivity to poly(adenosine diphosphate)-ribose polymerase inhibitorsBRCA1 CpG island hypermethylation predicts sensitivity to poly(adenosine diphosphate)-ribose polymerase inhibitors
Recently, Fong et al reported the antitumor activity of the poly(adenosine diphosphate)-ribose polymerase (PARP) inhibitor olaparib (AZD2281; KU-0059436) in patients with BRCA1/BRCA2 germline mutated ovarian cancer. Female ...
Vanadate increases L-type pyruvate kinase mRNAs level in adult rat hepatocytes in primary culture
In primary culture of adult rat hepatocytes, vanadate in the presence of glucose stimulates the expression of the liver (L-type) pyruvate kinase gene. Glucose by itself was inactive, and vanadate, like insulin, was also ...
Writers, readers and erasers of RNA modifications in cancer
Although cancer was originally considered a disease driven only by genetic mutations, it has now been proven that it is also an epigenetic disease driven by DNA hypermethylation-associated silencing of tumor suppressor ...
Mechanical properties of cultured human airway smooth muscle cells from 0.05 to 0.4 Hz.
We investigated the rheological properties of living human airway smooth muscle cells in culture and monitored the changes in rheological properties induced by exogenous stimuli. We oscillated small magnetic microbeads ...
Snail blocks the cell cycle and confers resistance to cell death
The Snail zinc-finger transcription factors trigger epithelial-mesenchymal transitions (EMTs), endowing epithelial cells with migratory and invasive properties during both embryonic development and tumor progression. During ...
DNA methylation-associated dysregulation of transfer RNA expression in human cancer
The human cytoplasmatic pool of tRNA for the 20 proteinogenic amino acids and selenocysteine is composed of 48 isoacceptor families -those tRNA with different anticodons- divided into 253 different isodecoder species -those ...
Micropatterning of single endothelial cell shape reveals a tight coupling between nuclear volume in G1 and proliferation
Shape-dependent local differentials in cell proliferation are considered to be a major driving mechanism of structuring processes in vivo, such as embryogenesis, wound healing, and angiogenesis. However, the specific ...
Actividades para promover la gestión crítica de la información de Internet aplicadas a estudiantes de farmacia
Con el fin de promover la adquisición de habilidades y destrezas que permitan al estudiante de Farmacia desarrollar una actitud activa y crítica frente a la información disponible en Internet, así como saber seleccionar ...
