Institut de Recerca Biomèdica (IRB Barcelona): Recent submissions
Now showing items 141-160 of 419
Centrosome Amplification Increases Single-Cell Branching in Post-mitotic Cells
Centrosome amplification is a hallmark of cancer, although we are still far from understanding how this process affects tumorigenesis [1, 2]. Besides the contribution of supernumerary centrosomes to mitotic defects, their ...
MD-DATA: the legacy of the ABC Consortium
The ABC Consortium has been generating nucleic-acids MD trajectories for more than 20 years. This brief comment highlights the importance of this data for the field, which triggered a number of critical studies, including ...
Systematic study of hybrid triplex topology and stability suggests a general triplex-mediated regulatory mechanism
By combining in silico, biophysical, and in vitro experiments, we decipher the topology, physical, and potential biological properties of hybrid-parallel nucleic acids triplexes, an elusive structure at the basis of life. ...
Gromologist: A GROMACS-oriented utility library for structure and topology manipulation
Despite the increasing automation of workflows for the preparation of systems for molecular dynamics simulations, the custom editing of molecular topologies to accommodate non-standard modifications remains a daunting task ...
Dysregulation of the FGF21-adiponectin axis in a large cohort of patients with severe obesity and liver disease
We investigated the impact of liver damage on systemic inter-organ communication in an extensive observational case-control study of 923 patients with severe obesity and biopsy-confirmed metabolic dysfunction-associated ...
Sensitive high-throughput single-cell RNA-seq reveals within-clonal transcript correlations in yeast populations
Single-cell RNA sequencing has revealed extensive cellular heterogeneity within many organisms, but few methods have been developed for microbial clonal populations. The yeast genome displays unusually dense transcript ...
Blood-brain barrier shuttle peptides: an emerging paradigm for brain delivery
Brain delivery is one of the major challenges in drug development because of the high number of patients suffering from neural diseases and the low efficiency of the treatments available. Although the blood-brain barrier ...
A single-cell resolved genotype-phenotype map using genome-wide genetic and environmental perturbations
Heterogeneity is inherent to living organisms and it determines cell fate and phenotypic variability. Despite its ubiquity, the underlying molecular mechanisms and the genetic basis linking genotype to-phenotype heterogeneity ...
Autocrine Wingless constricts the Drosophila embryonic gut by Ca+2-mediated repolarisation of mesoderm cells
Wg/Wnt signalling-a highly conserved transduction pathway-has most commonly been found to be involved in patterning, cell fate, or cell proliferation, but less so in shaping organs or body parts. A remarkable case of the ...
Proteomic tools for the quantitative analysis of artificial peptide libraries: detection and characterization of target-amplified PD-1 inhibitors
We report a quantitative proteomics data analysis pipeline which, coupled to protein-directed dynamic combinatorial chemistry (DDC) experiments, enables the rapid discovery and direct characterization of protein-protein ...
Size precision in insect eyes
The building of fully functional and well-proportioned individuals relies on the precise regulation of the size of each of their constituting organs. A new study unravels a mechanism that confers precision to size regulation ...
Aberrant phase separation and nucleolar dysfunction in rare genetic diseases
Thousands of genetic variants in protein-coding genes have been linked to disease. However, the functional impact of most variants is unknown as they occur within intrinsically disordered protein regions that have poorly ...
Understanding Retinoblastoma Post-Translational Regulation for the Design of Targeted Cancer Therapies
Simple Summary Rb1 is a regulator of cell cycle progression and genomic stability. This review focuses on post-translational modifications, their effect on Rb1 interactors, and their role in intracellular signaling in the ...
Multi-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming
The expression of the pluripotency factors OCT4, SOX2, KLF4, and MYC (OSKM) can convert somatic differentiated cells into pluripotent stem cells in a process known as reprogramming. Notably, partial and reversible reprogramming ...
BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis
Circadian rhythms control organismal physiology throughout the day. At the cellular level, clock regulation is established by a self-sustained Bmal1-dependent transcriptional oscillator network. However, it is still unclear ...
Origins of Second Malignancies in Children and Mutational Footprint of Chemotherapy in Normal Tissues
Pediatric cancers are rare diseases, and children without known germline predisposing conditions who develop a second malignancy during developmental ages are extremely rare. We present four such clinical cases and, through ...
Rhodium-Catalyzed Pauson−Khand Reaction Using a Small-Bite-Angle P‑Stereogenic C1‑Diphosphine Ligand
The asymmetric Pauson−Khand reaction catalyzed by [Rh(COD)(MaxPHOS)]BF4 is described. Several 1,6-enynes have been chosen as model substrates affording moderate yields and selectivities of up to 86% ee. Besides binap-type ...
CGeNArateWeb: a web server for the atomistic study of the structure and dynamics of chromatin fibers
We present CGeNArateWeb, a new web tool for the three-dimensional simulation of naked DNA and protein-bound chromatin fibers. The server allows the user to obtain a dynamic representation of long segments of linear, circular, ...
A chemo-centric view of human health and disease
Efforts to compile the phenotypic effects of drugs and environmental chemicals offer the opportunity to adopt a chemo-centric view of human health that does not require detailed mechanistic information. Here we consider ...
Optimal linker length for small molecule PROTACs that selectively target p38α and p38β for degradation
We report the design of hetero-bifunctional small molecules that selectively target p38α and p38β for degradation. These proteolysis targeted chimeras (PROTACs) are based on an ATP competitive inhibitor of p38α and p38β, ...
