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dc.contributor.author | Abate Daga, Daniel |
---|---|
dc.contributor.author | Andreu, Nuria |
dc.contributor.author | Camacho Sánchez, Juan |
dc.contributor.author | Alemany Bonastre, Ramon |
dc.contributor.author | Herance, Raúl |
dc.contributor.author | Millan, Olga |
dc.contributor.author | Fillat i Fonts, Cristina |
dc.date | 2018-11-30T10:05:26Z |
dc.date | 2018-11-30T10:05:26Z |
dc.date | 2011-10-18 |
dc.date | 2018-07-24T12:58:49Z |
dc.identifier | 22028820 |
dc.identifier.uri | http://hdl.handle.net/2445/126612 |
dc.description | Replication-competent adenoviruses armed with thymidine kinase (TK) combine the concepts of virotherapy and suicide gene therapy. Moreover TK-activity can be detected by noninvasive positron emission-computed tomography (PET) imaging, what could potentially facilitate virus monitoring in vivo. Here, we report the generation of a novel oncolytic adenovirus that incorporates the Tat8-TK gene under the control of the Major Late Promoter in a highly selective backbone thus providing selectivity by targeting the retinoblastoma pathway. The selective oncolytic TK virus, termed ICOVIR5-TK-L, showed reduced potency compared to a non-selective counterpart. However the combination of ICOVIR5-TK-L with ganciclovir (GCV) induced a potent antitumoural effect similar to that of wild type adenovirus in a preclinical model of pancreatic cancer. Although the treatment with GCV provoked a reduction in the viral yield, both in vitro and in vivo, a two-cycle treatment of virus and GCV resulted in an enhanced antitumoral response that correlated with high TK-activity, based on microPET measurements. Thus, TK-expressing oncolytic adenoviruses can be traced by PET imaging providing real time information on the activity of the virus and its antitumoral potency can be optimized by GCV dosing. |
dc.format | 11 p. |
dc.format | application/pdf |
dc.language | eng |
dc.publisher | Public Library of Science (PLoS) |
dc.relation | Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0026142 |
dc.relation | PloS One, 2011, vol. 6, num. 10, p. e26142 |
dc.relation | https://doi.org/10.1371/journal.pone.0026142 |
dc.rights | cc by (c) Abate Daga et al., 2011 |
dc.rights | http://creativecommons.org/licenses/by/3.0/es/ |
dc.rights | info:eu-repo/semantics/openAccess |
dc.subject | Adenovirus |
dc.subject | Càncer |
dc.subject | Adenoviruses |
dc.subject | Cancer |
dc.title | Oncolytic Adenoviruses Armed with Thymidine Kinase Can Be Traced by PET Imaging and Show Potent Antitumoural Effects by Ganciclovir Dosing |
dc.type | info:eu-repo/semantics/article |
dc.type | info:eu-repo/semantics/publishedVersion |