Antimicrobial and antioxidant effects of combined high pressure processingand sage in beef burgers during prolonged chilled storage

Abstract

The combined effect of sage (0.3 and 0.6%) and high pressure processing (HPP) [300 MPa (10 min, 9.9 °C) and 600 MPa (10 min, 10.2 °C)] on the antimicrobial and antioxidant characteristics of beef burgers during prolonged chilled storage (60 days) was analysed. Sage powder showed antioxidant and antimicrobial activities, but the addition of sage powder to burgers had no apparent effect on antimicrobial activity; however, antioxidant activity was detected as measured by TBARS, hexanal and photochemiluminescence (PCL). In general, lipid oxidation increased in all samples during storage. HPP at 600 MPa had no effect on lipid oxidation but caused mesophilic and psychrotrophic counts to remain close to the detection limit for at least 6 days. Significant correlations were found between lipid oxidation measured by TBARS and PCL and between TBARS with hexanal over the storage period. Sage had no detrimental effects on sensory attributes of burgers.

Industrial relevance Sage is an aromatic plant with excellent antimicrobial and antioxidant properties. High pressure processing (HPP) is an efficient non-thermal preservation technology. As far as the authors are aware, very few studies have holistically addressed the question of stability (microbial spoilage and oxidation of lipids) of traditionally-prepared burgers as affected by HPP and addition of a natural plant. This paper examines the possible application of both treatments so as to obtain beef burgers with suitable oxidative and microbiological stability over prolonged chilled storage without this affecting sensory attributes.